Evaluation of Genetic Variability among Three Pistacia Species Using Internal Transcribed Spacer 1 (ITS1) Marker

Diversity in Pistacia has been evaluated at all molecular levels using the internal transcribed spacer 1 (ITS1) marker three species (Pistacia atlantica subsp. atlantica; vera and terebinthus), compared with other species. Results showed that ITS amplification sequencing, followed by phylogenetic analyses, identify confirm their classification, which revealed it can be used as a marker. Our results suggest ITS1 analyses might provide simple inexpensive approach to validate of samples collected from natural population, where identification difficult, especially if hybrids are present or season is not optimal for identifying differences morphological traits.